CD41-KO mice are pancytopenic, and CD41-KO HSCs display increased apoptosis and proliferation. (A) Complete blood cytometry quantification of the platelet, erythrocyte, and leukocyte compartments in 2- to 3-month-old and 9- to 10-month-old mice. Leukocytes were lineage analyzed by FACS for markers of myeloid (Mac1), B-cells (B220), and T-cells (CD4/CD8a) (n = 12-19). (B) Whole-BM cellularity in 2- to 3-month-old and 6- to 12-month-old mice (n = 16-29). (C-D) FACS plots of BM myeloid (C) and B-lymphoid (D) markers. Scatter plots show total cellularity per femur of gated populations in 2- to 3-month-old and 12-month-old mice. (A-D) Pre-proB and pre-B (B220+IgM−) cells, Frs. (E-F) Immature B-cells (B220+IgMlo-high) (n = 5-14). Bar diagrams showing the composition (E) and total numbers per femur (F) of the LSK compartment in WT and CD41-KO mice (n = 8-10). (G) Levels of Annexin-V+7aad- apoptotic HSC subsets (n = 8-10). (H) In vivo BrdU incorporation assays of HSC subsets (n = 8-10). Error bars, SEM. *P < .05, **P < .01, ***P < .001 by Student t test. Frs, fractions; G, granulocytes (Mac1+Gr1+); M, monocytes/macrophages (Mac1+Gr1−).
