The hematopoietic defects of CD41-KO mice are LT transplantable and negatively affect WT hematopoiesis. (A) Noncompetitive transplantation setup: 500 000 WT or CD41-KO unfractionated BM were injected per recipient. (B) Donor chimerism vs endogenous cells shows near-total myeloablation of recipients (n = 5). (C) Complete blood cytometry analysis showing total leukocyte count in peripheral blood under steady-state conditions (0 months) and in recipient mice 1 and 4 months after BM transplantation with WT or CD41KO cells (n = 11-13). (D) Competitive transplantation setup: WT or CD41-KO BM cells were injected into recipients mixed at equal ratios (1:1; 200 000 + 200 000) with congenic WT BM competitor cells. (E) Donor chimerism level in peripheral blood of recipient mice at 1, 2, and 4 months after transplantation (n = 18-23). (F) Complete blood cytometry analysis of total peripheral blood showing (i) leukocyte and (ii) platelet numbers in recipients receiving either WT+WT or WT+KO cells (n = 4). Error bars, SEM. *P < .05, **P < .01, ***P < .001 by Student t test.