American Society of Hematology

Figure 7

$Figure 7. CD41:YFP+ LT-HSCs show robust, LT repopulation ability in secondary recipients and are more myeloid-biased than CD41:YFP− LT-HSCs. (A) Strategy of YFP+ and YFP− LT-HSC isolation and serial transplantation setup. Fifty to 100 LT-HSCs from 4- to 6-month-old CD41YFP/+ (CD41-HET) mice were transplanted with 200 000 competitor BM into lethally irradiated primary recipients. After 4 months, unfractionated BM was transplanted into lethally irradiated secondary recipients without addition of new competitor. (B-C) Top panels show donor chimerism in peripheral blood after 4 months in individual primary (B) and secondary (C) recipients. Bar colors: light gray, CD41:YFP−; dark gray, CD41:YFP+ donor HSCs. Bottom panels show donor lineage distribution in individual mice, corresponding vertically to the top panel bars.$

CD41:YFP+ LT-HSCs show robust, LT repopulation ability in secondary recipients and are more myeloid-biased than CD41:YFP− LT-HSCs. (A) Strategy of YFP+ and YFP− LT-HSC isolation and serial transplantation setup. Fifty to 100 LT-HSCs from 4- to 6-month-old CD41^YFP/+ (CD41-HET) mice were transplanted with 200 000 competitor BM into lethally irradiated primary recipients. After 4 months, unfractionated BM was transplanted into lethally irradiated secondary recipients without addition of new competitor. (B-C) Top panels show donor chimerism in peripheral blood after 4 months in individual primary (B) and secondary (C) recipients. Bar colors: light gray, CD41:YFP−; dark gray, CD41:YFP+ donor HSCs. Bottom panels show donor lineage distribution in individual mice, corresponding vertically to the top panel bars.

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