American Society of Hematology

Figure 4

$Figure 4. Impaired GC formation in Eμ-FOXP1 transgenic mice. (A) Analysis of the hematopoietic compartments in the spleen of unimmunized WT and Eμ-FOXP1 mice. Shown are representative FACS profiles (left) and a scatter plot summarizing the frequency of each cellular population relative to WT (right). Differences between young 2-month-old and older 7-month-old mice (circles) were assayed in 4 independent experiments. (B) The number of PNA+ GCs per mm2 of spleen (mean ± SEM) as measured by IHC from 2-month-old WT (n = 4) and Eμ-FOXP1 (n = 6) mice immunized with SRBCs. (C) Representative IHC staining with the proliferative marker PNA (brown) performed on splenic sections from SRBC-immunized mice. (D) Quantitative analysis of PNA+ surface area corresponding to 94 GCs from WT (n = 4) and 122 GCs from Eμ-FOXP1 (n = 6) 2-month-old immunized mice. (E) Shown are representative FACS profiles of splenic GC B cells after SRBC immunization (left) and the normalized percentage of GC B cells from WT and Eμ-FOXP1 mice (circles) assayed in 4 experiments (right). (F) IHC analysis of MZ CD21+ B cells (brown) in the spleen of WT and Eμ-FOXP1 SRBC-immunized mice (triangles). Shown are average size per mouse of the CD21+ area surrounding GCs (top) and representative IHC images (bottom). (G) Normalized FACS analysis of MZ CD21+CD23− B cells in the spleen from 2- to 8-month-old immunized WT and Eμ-FOXP1 mice (circles) assayed in 7 independent experiments. (H) Representative FACS profile and scatter plots of the normalized relative percentage of splenic B cells expressing different levels of surface IgM and IgD. WT and Eμ-FOXP1 2- to 8-month-old immunized mice (circles) were assayed in 4 independent experiments. Representative histograms of surface CD21 levels are shown for each fraction (in gray) compared with non-B B220− cells (in white). WT (solid lines) and transgenic (dotted lines) histograms were indistinguishable in terms of CD21 expression. The median is shown in all scatter plots. Significance was determined by nonparametric Mann-Whitney U tests.$

Impaired GC formation in Eμ-FOXP1 transgenic mice. (A) Analysis of the hematopoietic compartments in the spleen of unimmunized WT and Eμ-FOXP1 mice. Shown are representative FACS profiles (left) and a scatter plot summarizing the frequency of each cellular population relative to WT (right). Differences between young 2-month-old and older 7-month-old mice (circles) were assayed in 4 independent experiments. (B) The number of PNA⁺ GCs per mm² of spleen (mean ± SEM) as measured by IHC from 2-month-old WT (n = 4) and Eμ-FOXP1 (n = 6) mice immunized with SRBCs. (C) Representative IHC staining with the proliferative marker PNA (brown) performed on splenic sections from SRBC-immunized mice. (D) Quantitative analysis of PNA⁺ surface area corresponding to 94 GCs from WT (n = 4) and 122 GCs from Eμ-FOXP1 (n = 6) 2-month-old immunized mice. (E) Shown are representative FACS profiles of splenic GC B cells after SRBC immunization (left) and the normalized percentage of GC B cells from WT and Eμ-FOXP1 mice (circles) assayed in 4 experiments (right). (F) IHC analysis of MZ CD21⁺ B cells (brown) in the spleen of WT and Eμ-FOXP1 SRBC-immunized mice (triangles). Shown are average size per mouse of the CD21⁺ area surrounding GCs (top) and representative IHC images (bottom). (G) Normalized FACS analysis of MZ CD21⁺CD23⁻ B cells in the spleen from 2- to 8-month-old immunized WT and Eμ-FOXP1 mice (circles) assayed in 7 independent experiments. (H) Representative FACS profile and scatter plots of the normalized relative percentage of splenic B cells expressing different levels of surface IgM and IgD. WT and Eμ-FOXP1 2- to 8-month-old immunized mice (circles) were assayed in 4 independent experiments. Representative histograms of surface CD21 levels are shown for each fraction (in gray) compared with non-B B220⁻ cells (in white). WT (solid lines) and transgenic (dotted lines) histograms were indistinguishable in terms of CD21 expression. The median is shown in all scatter plots. Significance was determined by nonparametric Mann-Whitney U tests.

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