SMO activates NF-κB pathway independent of GLI1. (A) Silencing SMO in DOHH2 and HBL1 resulted in decrease of p-p65, (B) decrease of nuclear DNA binding activity of p65 and p50, and (C) decrease of total NF-κB luciferase activity. (D) Silencing SMO in HBL1 cells also resulted in decrease of 16 known direct NF-κB downstream target genes. The heat map was generated using TreeView software v1.1 (normalized, centered values). (E) Silencing PTCH in DOHH2 and HBL1 cells resulted in increase of p-p65. (F) No changes in p-p65 were seen after transiently silencing GLI1 by siRNA in DOHH2 and HBL1 or stably silencing GLI1 by shRNA in OCI-Ly19 cells. Data are presented as mean ± standard deviation of at least 2 independent experiments. P < .05.