SMO recruits and activates Gαi and Gα12 in DLBCL. (A) Immunoprecipitation (IP) assays showed that SMO coupled to Gαi, Gαq, and Gα12, but not to Gαs and Gα16 in HBL1 and BJAB. TC, total cell lysate; HC, heavy chain. Immunoprecipitation (B) and GTPase assays (C) showed that stimulation of SMO with recombinant Shh (250 ng/mL) for 10 minutes was associated with increased recruitment of Gαi and Gα12 to SMO as well as increased GTPase activity of Gαi and Gα12 in HBL1 and BJAB cells. Data are presented as mean ± standard deviation of 3 experiments. *P < .05.