Figure 1
![Figure 1. The region between CALM aa 520-583 is essential for CALM-AF10–mediated immortalization. Murine bone marrow progenitor cells were transduced with retroviral constructs expressing CALM-AF10 proteins schematically shown on the left. Bar graph (right) indicates the number of colonies generated per 10 000 cells seeded in second and third passage methylcellulose cultures. The mean ± SEM are shown from duplicate samples analyzed in 3 [CALM(1-552)-AF10], 4 [CALM(520-583)-AF10], or 5 [Vector, CALM-AF10, and CALM(1-583)-AF10] independent experiments.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/121/23/10.1182_blood-2012-06-435792/4/4758f1.jpeg?Expires=1735816095&Signature=aXzVerFLioVrvYY6pJO3QztrdHPuWlXFFNld7X7ytR3wGi5OUFxnbTg3BVKX74G6Zny9pZRpjiAXMFwY~06ONtmrp1C4WNpkE2xyLIaUiFKO8QlkPfkSYTU39k4yJBqoNrHjkM-f6zbHbP96brC1DJL-rWsrSdKHFY7cZI1EmTqfbUlrGQ95BKPVSb9Zkap78Wwlt9SAyGBy4La3FdvRwFOqlSDI4Q9xzviWU8WKIo9yw6ywqUfFlW3cDahvUkPgPec6MDr5xmqoh5CxM~b4JHzHHh9Y1LQYASmfBQHQbkstiY7LyrNrs~8X7EiiqF2WPxuX1i~IBP7tzThRpv~pjA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
The region between CALM aa 520-583 is essential for CALM-AF10–mediated immortalization. Murine bone marrow progenitor cells were transduced with retroviral constructs expressing CALM-AF10 proteins schematically shown on the left. Bar graph (right) indicates the number of colonies generated per 10 000 cells seeded in second and third passage methylcellulose cultures. The mean ± SEM are shown from duplicate samples analyzed in 3 [CALM(1-552)-AF10], 4 [CALM(520-583)-AF10], or 5 [Vector, CALM-AF10, and CALM(1-583)-AF10] independent experiments.
