Downregulation of the NF-κB signaling pathway components in NK cells from lymphoma patients. (A,B) NK cells were isolated from PBMCs of both healthy donors and lymphoma patients as described in the Materials and Methods section. The purified NK cells were then immediately subjected to RNA extraction and cDNA synthesis. The expression levels of p65 (A) and IRAK1 (B) were determined by SYBR Green real-time RT-PCR assay.