CD4⁺_SPT are more sensitive to IL-7 therapy than CD4⁺_PERI. (A) STAT5 phosphorylation in CD4⁺_PERI vs CD4⁺_SPT exposed to varying concentrations of rhIL-7. Results are representative of 3 independent experiments. (B) Quantification of bcl2 RNA expression on CD4⁺_PERI and CD4⁺_SPT exposed to varying IL-7 concentrations for 6 hours. Data show 1 mouse representative of 3 independent experiments. (C) Bcl-2 expression in CD4⁺_PERI vs CD4⁺_SPT exposed to varying concentrations of rhIL-7. (D) Representative flow cytometric analysis of IL-7Rα (CD127) and CD132 (common γC chain) evaluated on fresh CD4⁺CD8⁻ thymocytes (CD4⁺_SPT) and peripheral CD4⁺ T cells. Data are representative of 5 mice pooled from 3 independent experiments.