COX inhibitors reduce the percentages of lin^-Sca-1⁺c-Kit⁺ population in AE-expressing BM cells without inducing apoptosis. The lin⁻ BM cells from AE/Mx1-cre mice were cultured in methylcellulose-based medium in the presence of vehicle (DMSO), INDM (50 μM), or NS-398 (50 μM), and were replated as previously described. These cells were analyzed for cell cycle phases (A), apoptosis (B), and percentages of lin⁻Sca-1⁺C-Kit⁺ cells (C) by flow cytometry after 1 to 3 weeks of treatment. Two independent experiments showed similar results. The results of a duplicate experiment are shown in supplemental Figure 2.