The effect of blocking CXCL1/CXCL2 in vivo on the pattern of neutrophil recruitment. (A) Numbers of recruited neutrophils in peritoneal fluid of mice at 2 hours that were injected IV with anti-CXCL1, anti-CXCL2, or isotype control mAbs 15 minutes before IP injection either with LPS or PBS; mean ± SEM of n = 2 experiments with n = 4 mice per group. (B) Peritoneal wall sections immunostained for neutrophils (brown) from LPS-stimulated mice that were treated as in panel A. Representative images from n = 2 experiments with n = 4 mice per group. Quantification of neutrophils either (C) lining the peritoneal membrane as in panel B or (D) within the peritoneal wall tissue; n = 4 tissue sections.