The effect of macrophage depletion on neutrophil recuitment. (A) The effect of PBS- vs clodronate-liposome treatment on peritoneal macrophage and neutrophil numbers with/without LPS stimulation for 2 hours; n = 4 experiments with 4 to 8 mice per group in each experiment. (B) Representative tissue sections focusing on the peritoneal membrane and immunostained for neutrophils (brown) from LPS-stimulated mice that have received either PBS- or clodronate-containing liposomes. (C) Quantification of neutrophils lining the peritoneal membrane as in panel B. (D) Typical views of peritoneal wall tissue sections of PBS- and clodronate-liposome treated mice showing neutrophil distribution (brown). (E) Quantification of neutrophils within the peritoneal wall tissue as illustrated in panel D. Data from n = 3 experiments with 4 to 8 mice per group; A,C, and E are plotted as mean ± SEM.