Neutrophil recruitment in Mcpt5-Cre⁺iDTR⁺ mast cell–depleted mice. (A) Neutrophils recruited into the peritoneal fluid of Mcpt5-Cre⁺iDTR⁺ mice (black) compared with Cre-control mice (gray) post-LPS treatment or PBS treatment of Cre-control mice (white) at 1, 2, and 4 hours; mean ± SEM of n = 2 experiments, n = 5-7 mice per group, is shown. (B) Mast cells in peritoneal fluid in the same experiments as panel A. (C) Representative peritoneal wall tissue sections from Mcpt5-Cre⁺iDTR⁺ mice compared with Cre-control littermate mice highlighting neutrophil distribution (brown) 2 hours post-LPS treatment (representative of n = 5 samples for each tissue type). (D) Time course over 1, 2, and 4 hours of neutrophils recruited into peritoneal wall tissue after LPS stimulation of Mcpt5-Cre⁺iDTR⁺ mice (black) and Cre-control control mice (gray) or PBS treatment of Cre-control mice (white); n = 3 experiments with 3 to 6 mice per group. (E) Mast cells in peritoneal wall tissue in same experiments as panel D.