Characterization of PMN-derived MVs. (A) Fluorescence microscopy image of separated MVs. CD11b was marked with anti-CD11b R-PE conjugated monoclonal antibody. Original magnification is 630×. (B) Flow cytometry analysis of binding of FITC-conjugated annexin to phosphatidylserine. (C) Representative electron microscopic image of MVs. Original magnification is 10 000×. (D) Analysis of isolated MVs by dynamic light scattering. The x-axis is set to logarithmic scale, a(r_h) denotes the coefficient of autocorrelation function of the scattered electric field. (E) Superoxide production of 10⁶ PMNs or MVs derived from 10⁷ PMNs stimulated by 100nM PMA (dots show mean of measured RFU ± SEM, n = 4).