Change in frequency of GI indicator clones between diagnosis and day +30 after diagnosis. (A) SR patients but not PR patients show a longitudinal expansion of GI indicator clones in the blood (top 100 clones in GI tissue). Shown are all GI indicator clones that were detected in blood, reported as ratio fold change of indicator clones at day +30 after diagnosis compared with time of diagnosis. As measured by ICI-30, SR patients show a statistically significant expansion of GI indicator clones when comparing (B) tissue samples or (C) averaged tissues per patient. (D) Control ICI-30 values calculated by defining indicator clones as the 100 most frequent clones in blood at time of diagnosis are not different between PR and SR patients.