Human DC subsets in huNSG mice. (A) Flow cytometric staining of CD11c⁻, CD303⁺ pDCs, or CD1c⁺ and CD141⁺ on CD11c⁺ cDCs of the spleen (left). Samples were pregated as singlet, live cells positive for hCD45 and HLA-DR and negative for lineage, CD14, and CD16. Percentages of those subsets in relation to live, singlet, human CD45⁺ cells are shown in the middle. The numbers on top of the data points indicate the average percentage of human CD45-positive cells for each analyzed DC subset. Absolute numbers of DC populations are shown on the right. Graphs represent data from mice from 4 independent reconstitutions. Each data point represents one individually analyzed mouse. (B) Same as in panel A for DC subsets of the BM. (C) Same as in panel A for DC subsets of the blood. (D) Immunofluoresence microscopy for DC markers on spleen sections. DEC-205 in red as DC-marker, CD20 in green as B-cell marker, and CD3 in blue as T cell marker.