αDEC-A647 is taken up most efficiently by CD141⁺ cDCs in vivo. (A) 5 μg of αDEC-A647 or IgG-A647 was injected intraperitoneally into huNSG mice. After 3 hours, the mice were euthanized, and antibody uptake into the different cellular subsets in the spleen was analyzed by fluorescence-activated cell sorting. (B) Same as in panel A but shown for mice from 2 independent experiments, including 3- and 5-hour time points, which showed similar results. Each data point represents one individually analyzed mouse. Statistical analysis was performed with paired t tests.