Characterization of p65 hematopoietic null mice. (A) Breeding scheme for the generation of p65hem−/− mice. p65 expression in (B) whole bone marrow cells or (C) HSCs (lineage-Flk2−Sca-1+c-kit+ fraction) of p65hem−/− or VCre+ (wild-type) mice was analyzed by quantitative reverse transcriptase-polymerase chain reaction. (D) Whole bone marrow or (E) whole splenocytes from p65hem−/− or wild-type mice were analyzed for p65 protein expression.