p65 deletion results in deregulation of hematopoietic stem and progenitor cell homeostasis. Bone marrow cells from wild-type or p65hem−/− mice were analyzed for the frequency of (A) LSK cells and (B) HSPCs by flow cytometry (n ≥ 7). (C) The average percentage of cells is displayed. Bone marrow cells from wild-type or p65hem−/− mice were analyzed for (D) myeloid (n ≥ 7) or (E) lymphoid (n = 4) progenitor cell frequency using flow cytometry. (F) The average percentage of cells is displayed. (G) The average total number of cells recovered from the femurs and tibias of mice from each genotype is displayed (n ≥ 10). (H) Femur sections were stained with hematoxylin and eosin or reticulin to monitor changes in morphology or fibrosis. Images are representative of staining from 3 different animals per genotype. *P ≤ .005.
