PHD2-deficient hematopoietic progenitors are outcompeted by WT littermate cells. (A) Schematic overview of the competitive BM transplantation model in lethally irradiated CD45.1 recipient mice. All mice were bled every 4 weeks and PB chimerism was measured via FACS. Final analysis on BM cells was performed 16 weeks after injection. (B) Representative FACS plots showing PB chimerism 16 weeks after transplantation. Graphical illustration at the indicated times after BM transfer representing the percentage of donor-derived CD45.2 cells (WT or cKO) (n = 5). (C-D) The absolute number of CD45.2 WT and cKO BM cells (C) with or (D) without CD45.1 WT competitor BM cells, 16 weeks after BM transfer (n = 5). (E-F) The absolute number of WT and cKO in the (E) LSK compartment and (F) subdivided in the 5 HSC/MPP (n = 5). (G) Homing of CFSE-labeled Lin⁻ cells to BM from WT or cKO mice 20 hours after transfer (n = 5). Abs. cell n°, absolute cell number.