HIF1α induces self-renewal of PHD2-deficient HSCs/MPP1 during transplantation stress. (A) Schematic overview of the whole-BM–transplantation mouse model in lethally irradiated CD45.1 recipient mice. Analysis was performed 16 weeks after intravenous injection of BM cells. (B-C) The absolute number of WT and cKO (B) total BM and (C) LSK cells in recipient mice. Representative FACS plots (D) from a WT or cKO BM recipient with the latter (E) showing significantly more cells in HSC/MPP1 compartment (n = 5). (F) The absolute number of WT and cDKO1 total BM LSK cells in recipient mice subsequently subdivided in the 5 HSC/MPP subsets showing no significant difference in any of the investigated compartments (n = 5-7). Abs. cell n°, absolute cell number.