Outcomes after treatment with banked VSTs: CMV-infected patients. (A) Depiction of viral load measurements before and during 6 weeks postinfusion of VSTs in 19 evaluable patients (responders and nonresponders) treated for persistent CMV infection. Results are presented as copies per milliliter (CMV detected in blood) or as copies per cell in patients with CMV antigenemia. Because viral loads were measured by different assays at different institutions, interpatient comparisons should not be made. (B) An example of a patient with CMV colitis with evidence of viral inclusions on H&E staining that contained CMV by immunostaining (left panel). After VST infusion, there was a complete resolution of CMV colitis and no evidence of viral inclusions by H&E staining (right panel). (C) Frequency of CMVpp65–directed T cells in peripheral blood before and at weeks 1, 2, 4, and 12 postinfusion, as measured with an IFN-γ ELIspot assay after overnight stimulation of PBMCs with CMVpp65 pepmix. Results are expressed as mean (±SD) spot-forming cells (SFCs) per 2 × 105 input cells.
