In vivo cell spreading assay. (A) Visualization of CHOβ3 cells with expression of SH31-141, SH31-141G116R, or SH31-141G127R. The expression of integrin β3 (green) and the SH3 protein (red) was detected using antibodies against integrin β3 and Flag-tag, respectively, and visualized under confocal microscopy. (B) A statistics analysis of cell spreading assay. The surface areas of adherent cells were measured using ImageJ software (National Institutes of Health). The averaged cell area for each coexpression was calculated over a dataset containing 60 randomly chosen cells. Values are mean ± SE; n = 60. **P < .01, statistically significant compared with control CHOβ3 cells. ***P < .001, statistically significant compared with control CHOβ3 cells.
