Internalization of VWF by CLEC4M-expressing cells. (A) Internalization of VWF by CLEC4M-expressing cells was confirmed by demonstrating colocalization of VWF (green) and early endosomal antigen 1 (red). Blue represents the DAPI nuclear counterstain. (B) Incubation of CLEC4M-expressing cells (red) at 4°C maintained VWF (green) at the cell surface, whereas incubation at 37°C facilitated internalization of VWF.