Accelerated maturation of BM-derived neutrophils in Wip1-deficient mice. (A) The maturation of granulocytes in BMCs can be distinguished by the expression of CD11b/Gr-1. Three populations can be achieved: CD11b^intGr-1^int cells (promyelocyte/myelocyte neutrophils), CD11b^hi Gr-1^hi cells (mature neutrophils), and CD11b^lowGr-1^hi cells (immature neutrophils). One representative FACS analysis of BM CD11b⁺Gr-1⁺ neutrophils was shown. (B) The neutrophil maturation phenotype of WT or Wip1KO mice was analyzed on freshly isolated and erythrocyte-depleted BMCs. Represented is one of 5 independent experiments with similar results. The percentage of cells in the different developing stages among CD11b⁺Gr-1⁺ cells in full (C) or mixed chimeric (D) mice were analyzed by FACS. Chimeras were generated by transplanting either CD45.2⁺ WT or Wip1KO BM into CD45.1⁺ lethally irradiated mice as described in “Methods.” Data are mean ± SD and are representative from 3 independent experiments. *P < .05, compared with WT controls. **P < .01, compared with WT controls. ***P < .001, compared with WT controls.