EpoR silencing in HEL-Lu cells does not affect JAK2/Akt signaling. (A) Flow cytometry analysis of EpoR expression in 2 HEL-Lu shEpoR clones. The percentage of EpoR-positive cells is indicated for both clones (black bold curves) and for nontransduced cells (NT; gray thin curves). Dotted lines represent the IgG-isotype control. (B) Typical microscopy images showing HEL-Lu and HEL-Lu shEpoR adhesion to laminin at 2 dyn/cm². (C) Quantification of active Akt in HEL-Lu shEpoR cells (clones #2 and #26) and HEL-Lu cells in the absence (UT) or presence of different inhibitors. Unpaired t test, **P < .01 and ***P < .001, compared with UT (n = 4).