Differences in the NK-cell receptor repertoire in NKG2D-deficient mice. (A) Representative FACS histograms and (B) percentages of splenic NK cells expressing Ly49D, Ly49C/I, Ly49G2, Ly49F, and Ly49A are shown for 5 mice per genotype. Data are representative of at least 5 independent experiments. (C) Representative FACS histograms depicting DNAM-1 expression on splenic NK cells (CD3-gfp+), NKT cells (CD3^lo, NK1.1+), and CD8+ T cells from 1 mouse of each indicated genotype (representative of at least 10 individual mice/genotype). (D) Percentages of DNAM-1–expressing NK cells in BM, spleen, and liver of 5 mice per genotype. Data are representative of at least 4 individual experiments.