NK-cell resistance to MCMV infection is not enhanced in the absence of NKG2D. (A) B6 NKG2D-KO and WT littermates were treated with anti-NK1.1 or control IgG before infection with MCMV (1 × 106 PFU intraperitoneally); virus titers were assessed in the liver, spleen, and lungs 4 days post infection. Data are representative of 3 independent experiments. (B) Groups of WT mice were treated with 250 μg of anti-NK1.1 (PK136), anti-NKG2D (MI-6), or control IgG before infection with MCMV (3 × 106 PFU intraperitoneally) of MCMV; virus titers in the spleen and liver were assessed 3 days post infection. Data are representative of 2 independent experiments. All results are expressed as geometric mean ± SD of 5 mice per group. (C) Representative FACS profile of Ly49H expression on NK cells from WT and NKG2D-KO mouse. (D) Percentages of IFN-γ–producing NK cells upon stimulation with anti-Ly49H at the indicated concentrations. Data are representative of 2 of 3 independent experiments.
