Depletion of lysosomes in fibroblasts. Control (A-C) and patient V-11 (D-F) fibroblasts were grown in regular medium (A,D) or starvation medium (B-C,E-F) and stained with LysoTracker (red) with (C,F) or without (A-B,D-E) subsequent MitoTracker (green) stain. Cells were visualized live by fluorescent confocal microscopy without (C,F) or with differential interference contrast (A-B,D-E) at ×400 magnification.