Increased apoptosis in bone marrow. (A) Immunostaining of activated caspase-3 in the bone marrow showed markedly increased apoptosis. The image is representative of 3 patients. (B) Similarly stained normal bone marrow of a patient of the same age is shown. A biopsy was obtained from a patient with Wilms tumor in order to assess the presence of metastases. (C) Increased apoptosis of granulocytes in the bone marrow of patients with mutated VPS45 patients is shown. Bone marrow sections of a healthy person and 3 patients with VPS45 mutations were co-immunostained with specific antibodies against activated caspase-3 (green) and CD177 (red). DNA was counterstained using DAPI.