Differential expression of candidate markers between leukemic blasts and B-cell subsets from nonmalignant controls. Shown are MFI values measured for indicated markers in malignant populations of newly diagnosed leukemia samples (ALL, n = 34) and in subpopulations representing different B-cell maturation stages (CD10⁺ CD20^–; CD10⁺ CD20⁺; and CD10^– CD20⁺) from normal bone marrow and remission samples with no evidence of disease (n = 20). Samples from postinduction therapy time point (day 78) are labeled in red. MFI values measured for cell population size <100 events were excluded. Each point represents a single measurement, and gray bars represent the median value for the group. The asterisk indicates a statistically significant difference in marker expression by the Mann-Whitney test with specified P value.