Identification of ZNF24-binding site within the proximal VEGF promoter. (A) EMSA assays were performed using the −161/−83 32P-labeled probe and indicated cold oligonucleotide competitors. (B) Representation of the 32P-labeled probes and cold oligonucleotide competitors used in EMSA assays. (C-D) EMSA assays were performed using the indicated 32P-labeled probes and cold oligonucleotide competitors. (E) The EMSA assay was performed using the −150/−128 fragment as the probe with the indicated Abs for immunodepletion. (F) Control plasmid or the ZNF24 expression plasmid was cotransfected with full-length VEGF promoter luciferase construct along with scrambled ODN or VEGF promoter ODN into MDA-MB-231 cells. *P = .04. NS indicates not significant. (G) The indicated ODNs were transfected into MCF7 cells and real-time PCR was performed using primers specific for VEGF and GAPDH. *P = 1.83 × 10−5.
