Figure 5
Figure 5. CD8 T cells of Stx11−/− mice display features of a final stage of exhaustion. (A-B) Stx11+/− (○) and Stx11−/− (●) mice were infected intravenously with 200 PFU LCMV. On day 36 (A) and day 180 (B), the frequencies of LCMV-specific CD8 T cells were determined by GP33-tetramer staining. IFN-γ production of CD8 T cells was analyzed after 4 hours of in vitro restimulation with GP33 peptide. FACS illustrations are representative for the respective mouse groups; graphs represent data for individual mice. (C) IFN-γ expression levels of GP33-tetramer-positive CD8 T cells from Stx11+/− (○) and Stx11−/− (●) mice were determined by mean fluorescence intensity after short-term restimulation on days 12, 36, and 180 after infection. Horizontal lines in graphs represent mean values. Data (mean ± SEM) represent at least 2 independent experiments with 2 or 3 mice per group. **P < .005 (Student unpaired t test). ***P < .001 (Student unpaired t test).

CD8 T cells of Stx11−/− mice display features of a final stage of exhaustion. (A-B) Stx11+/− (○) and Stx11−/− (●) mice were infected intravenously with 200 PFU LCMV. On day 36 (A) and day 180 (B), the frequencies of LCMV-specific CD8 T cells were determined by GP33-tetramer staining. IFN-γ production of CD8 T cells was analyzed after 4 hours of in vitro restimulation with GP33 peptide. FACS illustrations are representative for the respective mouse groups; graphs represent data for individual mice. (C) IFN-γ expression levels of GP33-tetramer-positive CD8 T cells from Stx11+/− (○) and Stx11−/− (●) mice were determined by mean fluorescence intensity after short-term restimulation on days 12, 36, and 180 after infection. Horizontal lines in graphs represent mean values. Data (mean ± SEM) represent at least 2 independent experiments with 2 or 3 mice per group. **P < .005 (Student unpaired t test). ***P < .001 (Student unpaired t test).

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