The 3q21q26 and 3q21q26::Gata2^+/−leukemia cells exhibit 2 distinct differentiation patterns. (A) Percentages of B220⁺Gr1^– cells vs Gr1⁺ cells (B220⁺Gr1⁺ cells plus B220^–Gr1⁺ cells) in bone marrows of the leukemic 3q21q26 (left panel [n = 60]) and 3q21q26::Gata2^+/− (right panel [n = 32]) mice. We classified the leukemias into 4 types; Type I, II, III, and Unclassified based on the percentages of B220⁺Gr1^– cells and Gr1⁺ (ie, B220⁺Gr1⁺ and B220^–Gr1⁺) cells. The yellow-shaded area in both graphs indicates a range in which more than 80.0% of the leukemic bone marrow cells are both B220⁺ and Gr1⁺. The numbers of mice categorized into each type and their percentages are shown below the graphs. (B) Representative flow cytometric profiles of the bone marrows from Type I, II, III, and Unclassified leukemic 3q21q26 mice. (C) Representative smears of peripheral blood taken from Type I, II, III, and Unclassified leukemic 3q21q26 mice. (D) Percentages of c-Kit-positive cells in the bone marrow of the 3q21q26 Type I (n = 16), II (n = 18), III (n = 20), and Unclassified (n = 6) leukemia mice and the 3q21q26::Gata2^+/− Type I (n = 12), II (n = 2), III (n = 5), and Unclassified (n = 10) leukemia mice. (E) Kaplan-Meier survival curves of WT mice transplanted with 3q21q26 Type I (n = 20; median, 28.0 days), II (n = 31; median, 38.5 days), and III (n = 28; median, 69.0 days) or 3q21q26::Gata2^+/− Type I (n = 36; median, 23.5 days) leukemic whole bone marrow cells (0.5 to 1 × 10⁶ cells).