Knockout of Psip1 reduces peripheral blood cell counts. (A-B) qRT-PCR of lineage-depleted BM cells (A) and western blot of whole BM cells (B) validating complete LEDGF/p75 depletion in Psip1 knockout (Psip^Vav/Vav) mice compared with the floxed control mice (Psip^Fl/Fl). Messenger RNA (mRNA) expression in (A) and protein expression in (B) were normalized to Gapdh and β-tubulin, respectively. (C-D) Peripheral blood cell counts measured in Psip^Fl/Fl and Psip^Vav/Vav mice (8-12 weeks of age). Mean values and standard error of the mean (SEM) are indicated. HGB, hemoglobin; RBC, red blood cells; ns, nonsignificant. (E) Total cell number extracted from spleens and thymuses of Psip^Fl/Fl and Psip^Vav/Vav mice (8-10 weeks of age). Mean values and SEM are indicated. P values (*) show significance (**P < .01, ***P < .001, Student t test).