Quantification of putative stem cell niche markers in different steady-state BM compartments. (A) Diagram displaying the process of in vivo fluorescence trapping. For each sample, total fluorescence intensity and number of cells were measured ex vivo to calculate the fluorescence intensity per 106 cells. (B) Quantification of BRA using NIR-pamidronate in vivo fluorescence trapping. Average values from 4 animals ± SD are displayed (n = 2 experiments). (C) Quantification of BVF using NIR blood pool agent in vivo fluorescence trapping in identical conditions as previously, except a 10- to 15-minute incubation after in vivo administration. Data are average values from 6 animals (3 experiments) ± SD. *P < .05 or **P < .005. (D) Quantitative reverse-transcription–PCR quantification of osteoclast (cathepsin K), osteoblast (osteocalcin), and endothelial cell (VE-cadherin and VEGFR2) specific mRNAs. CT values used were the result of 2 different duplicates from 4 independent experiments.
