Activation status of Tregs and Tconvs in different healthy human tissues. The activation status of human CD4^pos Tregs and Tconvs as indicated by CD45RA and CD69 expression was analyzed by flow cytometry on PB, BM, liLNs, inLNs, and SPL. (A) Representative examples of CD45RA and CD69 staining on expression on CD4^posCD25^posCD127^neg Tregs and CD4^posCD25^negCD127^pos Tconvs. (B) Summary of distributions of Tregs and Tconvs over 4 activation stages, based on expression of CD45RA and CD69. Data are depicted as percentages of cells within each stage. Each line represents 1 sample (N = 10-14 for each tissue). Data were compared on the log-odds scale using a mixed model, and significant differences are indicated: *P < .05; **P < .01; ***P < .001.