Immunohistochemistry of FoxP3^poscells in human secondary lymphoid organs. Immunohistochemistry of representative specimens of human liLNs, inLNs, and SPL samples. (A) Anti-FoxP3 (magenta) and anti-CD69 (blue) staining (magnification, ×20). (Left panel) Photo image. (Right panel) Composite image with pseudocolor: FoxP3^posCD69^pos (red) and FoxP3^posCD69^neg (green). (B-C) Anti-FoxP3 (brown) and anti-Aire (blue) (B) or anti-CD11c (blue) (C). (Left panel) Photo image. (Right panel) Composite image with pseudocolor: FoxP3^pos (black). A representative digital magnification of the composite image procedure to generate pseudocolors in ImageJ is shown on the bottom.