Notch1 loss rescued aberrant lymphoid fate determination and HSC defects in Lrf knockout mice. (A) Representative FACS profiles of BM B-cell compartments in control, Lrf knockout (Zbtb7aFlox/FloxMx1-Cre+), Notch1 knockout (Notch1Flox/FloxMx1-Cre+), and Lrf/Notch1 double-knockout (Zbtb7aFlox/FloxNotch1Flox/FloxMx1-Cre+) mice 1 month after the last pIpC injection. (B) Proportions of DP T (top) and pre-B cell compartments (bottom) in BM. Horizontal black bars: average value; error bars: standard deviation. (C) Numbers of LT-HSCs recovered in Lrf knockout mice on Notch1 loss. Dot graph shows absolute numbers of LT-HSCs per 2 legs in indicated mice 1 month after the last pIpC injection. Horizontal black bars: average value; error bars: standard deviation. (D) LT-HSCs numbers recover normally after 5-FU challenge in Lrf/Notch1 double-knockout mice. Dot graph shows absolute numbers of LT-HSCs per 2 legs in indicated mice 3 weeks after 5-FU treatment. Horizontal black bars: average value; error bars: standard deviation. (E) Survival curves after sequential 5-FU treatment. 5-FU (100 mg/kg) was administered weekly. Survival rates in each group were analyzed and P value calculated using a log-rank Mantel-Cox test.
