Figure 1
Figure 1. Treg responses in immunotherapy-treated and nontreated infected mice. (A) Flow cytometry analyses of Treg responses in mice 8 weeks of age. Mice were infected neonatally with FrCasE. Infected mice were immunotherapy treated shortly after infection using repeated administration of 667, 667-F(ab′)2, or 672 for 5 days (see “Methods”) and compared with control noninfected/nontreated and infected/nontreated animals. Treg levels were assayed by flow cytometry 8 weeks later on the basis of CD4, CD25high, and FoxP3 positivity and were expressed as a percentage of total CD4+ T cells. (B) Statistical analysis of Treg responses in mice 8 weeks of age. Three experiments comprising 3 mice each were conducted as described in panel A. The data are presented as means ± SEM of Tregs expressed as a percentage of total CD4+ T cells. The statistical significance between groups was established using the Student t test. **P < .01. (C) Treg development in the thymi, spleens, and lymph nodes of noninfected/nontreated, infected/nontreated, and infected/667-treated mice. The different groups of mice were infected and treated as in panel A. Tregs were assayed as in panels A and B in total CD4+ cells recovered from each organ. Data are presented as means ± SEM of 3 independent experiments conducted with 2 mice per time point. (D) Infection kinetics in the thymi, spleens, and lymph nodes of infected/nontreated mice. Mice were infected and cells were then prepared at various time points, from the thymi, spleens, and lymph nodes for flow cytometry quantification of Env expression at their surface, as described in “Methods.” Values are the means ± SEM of 3 independent experiments conducted with 3 mice per condition. (E) Effect of FrCasE viral challenge on Treg levels in infected/nontreated mice. Mice were infected and subjected to viral challenge 8 weeks later, as described in “Methods,” and Treg levels were assayed after another 8 days, as in panels A and B. Values are the means ± SEM of 3 experiments conducted with 3 mice each. The statistical significance between groups was established using the Student t test. *P < .05.

Treg responses in immunotherapy-treated and nontreated infected mice. (A) Flow cytometry analyses of Treg responses in mice 8 weeks of age. Mice were infected neonatally with FrCasE. Infected mice were immunotherapy treated shortly after infection using repeated administration of 667, 667-F(ab′)2, or 672 for 5 days (see “Methods”) and compared with control noninfected/nontreated and infected/nontreated animals. Treg levels were assayed by flow cytometry 8 weeks later on the basis of CD4, CD25high, and FoxP3 positivity and were expressed as a percentage of total CD4+ T cells. (B) Statistical analysis of Treg responses in mice 8 weeks of age. Three experiments comprising 3 mice each were conducted as described in panel A. The data are presented as means ± SEM of Tregs expressed as a percentage of total CD4+ T cells. The statistical significance between groups was established using the Student t test. **P < .01. (C) Treg development in the thymi, spleens, and lymph nodes of noninfected/nontreated, infected/nontreated, and infected/667-treated mice. The different groups of mice were infected and treated as in panel A. Tregs were assayed as in panels A and B in total CD4+ cells recovered from each organ. Data are presented as means ± SEM of 3 independent experiments conducted with 2 mice per time point. (D) Infection kinetics in the thymi, spleens, and lymph nodes of infected/nontreated mice. Mice were infected and cells were then prepared at various time points, from the thymi, spleens, and lymph nodes for flow cytometry quantification of Env expression at their surface, as described in “Methods.” Values are the means ± SEM of 3 independent experiments conducted with 3 mice per condition. (E) Effect of FrCasE viral challenge on Treg levels in infected/nontreated mice. Mice were infected and subjected to viral challenge 8 weeks later, as described in “Methods,” and Treg levels were assayed after another 8 days, as in panels A and B. Values are the means ± SEM of 3 experiments conducted with 3 mice each. The statistical significance between groups was established using the Student t test. *P < .05.

Close Modal
This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal