Quantification of cell-associated HIV DNA in pathogen-specific CD4 T cells after in vitro HIV infection. (A) Pathogen-specific CD4 T cells were sorted from PBMCs with a FACSAria cell sorter based on CFSE_low. (B) Quantification of cell-associated strong-stop and full-length HIV DNA in sorted pathogen-specific CD4 T cells. The results are expressed as the fold increase in HIV DNA copies for TT- and C albicans–specific CD4 T cells relative to CMV-specific CD4 T cells (mean and SD). Three independent experiments for each donor with a total of 3 donors were performed. Statistical analysis was performed using the Mann-Whitney test. *P < .05.