Damage of LYVE-1+ lacteals occurs before damage to the surrounding blood capillaries and to the whole architecture of the villi, and the lymphatic vessels in Peyer patches disappear in the LYVE-1-Cre/iDTR mice after DT administration. Control and LCiDT mice were given DT (100 ng), and their intestines were harvested at indicated times and analyzed. (A) Images showing LYVE-1+ lacteals and CD31+ blood vessels in the villi of the jejunum at 6, 12, and 24 hours after DT administration. (B) Quantification of the LYVE-1+ area and the CD31+ area in the intestinal villi. Each group, n = 4. *P < .05. (C) Images showing LYVE-1+ lacteals and E-cadherin+ epithelial cells in the intestinal villi at 24 hours after DT administration. (D) Images showing LYVE-1+ lymphatic vessels and CD31+ blood vessels in the Peyer patches. Indicated areas (square with white dotted line) are magnified in the right panel. The arrowhead indicates lymphatic vessels located at the serosal side of the Peyer patch. (E) Quantification of the LYVE-1+ area in the Peyer patches. Each group, n = 4. *P < .05. All images were stained with 4,6 diamidino-2-phenylindole (DAPI) for nuclei imaging. Scale bars, 50 µm.