No apparent phenotypes are detected in BM-LCiDT mice at 24 hours after DT administration. (A) Comparisons of flow cytometric profiles of LYVE-1+/CD45+ macrophages, CD3+/CD45+ T lymphocytes, B220+/CD45+ B lymphocytes, CD11b+/F4/80+ macrophages, and CD11b+/F4/80− monocytes in the peripheral bloods. Note that there are no differences in the population of each immune cell between BM-iDT and BM-LCiDT. (B) Gross images of the abdominal contents. The arrow indicates the proximal portion of the small intestine, and the arrowhead indicates the Peyer patch. (C) Intestinal villi in the duodenum stained with hematoxylin and eosin. Scale bars, 100 μm. (D) LYVE-1+ lymphatic vessels and CD31+ blood vessels in jejunums. Scale bars, 100 μm. Similar findings were observed in 3 to 4 different mice. (E) Blood profiles of hemoglobin, hematocrit, neutrophils, lymphocytes, and platelets. Each group, n = 4 to 5. DAPI, 4,6 diamidino-2-phenylindole; H&E, hematoxylin and eosin.