Figure 1
HSPCs contribute to the innate immune response during cutaneous S aureus infection. Lys-EGFP mice were treated with a mAb to c-kit to deplete BM HSPC numbers, or to an isotype control antibody. Full-thickness skin wounds 6 mm in diameter were inoculated with 1 × 107 CFUs of a bioluminescent S aureus strain. (A) Kinetics of EGFP-PMN fluorescence after wounding and inoculation at day 0. (B) S aureus bioluminescent signal and (C) wound size as the percentage of day 0 value all measured using whole animal fluorescence imaging of live animals for 7 days after wounding. Data represent 5 to 8 mice per group and are expressed as mean ± standard error of the mean (SEM) (*P < .05).
