Numbers of HSPCs and their progeny in BM and cutaneous wounds infected with S aureus. (A) Full-thickness skin wounds (6 mm) were created on the backs of WT mice and were inoculated with 1 × 10⁷ CFUs of S aureus (triangles) or saline vehicle control (circles). Lineage-negative, c-kit⁺ HSPCs in BM and skin wound digests were evaluated via flow cytometry on days 1, 3, and 5 after wounding. (# indicates significant difference compared with day 0, P < .05; *significant difference compared with saline, P < .05) (B-C) Populations of PMNs and their progenitors in skin wounds (B) and BM (C) of WT mice were measured via flow cytometry 3 days after wounding. LSK refers to lineage-negative, Sca-1⁺, c-kit⁺ cells, a population enriched for hematopoietic stem cells; CMPs, common myeloid progenitor cells; bands and PMNs, immature banded and mature segmented neutrophils. (**significant difference compared with saline, P < .01) Data represent 8 mice per group during 3 experiments and are expressed as mean ± SEM.