Ligand sensitivity was associated with outcome. (A) Kaplan-Meier survival curves demonstrate that patients whose blasts were sensitive to G-CSF (log₂(ΔMFI) ≥ 1 at 1 or 10 ng/mL dose) had a superior EFS (left) and OS (right) compared with patients whose blasts did not respond at the lower dose levels (G-CSF resistant). (B) The sensitivity of blasts to both G-CSF and IL-6 also significantly predicted EFS (left) and OS (right). Group SS indicates G-CSF sensitive and IL-6 sensitive (log₂(ΔMFI) ≥ 1 at dose level 2); group SR indicates G-CSF sensitive and IL-6 resistant; group RR indicates G-CSF resistant and IL-6 resistant; and group RS indicates G-CSF resistant and IL-6 sensitive.