Analysis of erythropoietic parameters in treated Hfe^−/− mice. The RBC parameters hemoglobin (Hgb; A), mean cell volume (MCV; B), and reticulocyte mean cell hemoglobin (CHr; C) were measured in female WT and Hfe^−/− using the same treatment schedule as described in Figure 2. Data are presented as mean ± SEM (n = 5 for each genotype.) P values were calculated using the Student t test. ****P < .001; ***P < .005; **P < .01; *P < .05.