Analysis of erythropoietic parameters in treated Hbb^th3/+ mice. (A,C,D) The RBC parameters Hgb (A), MCV (C), and reticulocyte mean cell hemoglobin (CHr; D) were measured in female WT and Hbb^th3/+ mice. (B) ELISA erythropoietin analysis of serum collected from WT and Hbb^th3/+ mice on same treatment schedule as described in Figure 4 (n = 5 or 6 for each genotype). Data are presented as mean ± SEM. P values were calculated using the Student t test. ****P < .001; ***P < .005; **P < .01; *P < .05.