Analysis of RBC maturation in treated Hbb^th3/+ mice. (A-C) Total spleen weight (g; A), RBC count (B), and reticulocyte count (Retic; C) are depicted. (D) Murine RBCs were biotinylated and flow cytometric analysis of streptavidin-labeled cells was completed every 7 days (WT LNP-Luc, n = 5; WT LNP-Tmprss6, n = 4; Hbb^th3/+ LNP-Luc, n = 2 or Hbb^th3/+ LNP-Tmprss6, n = 3) to determine RBC survival. (E) RBC membrane-bound globins were analyzed using TAU gel electrophoresis. The number below each lane is the intensity of α-globin compared with β-globin. Globins from the WT-soluble fraction were used as a standard. Data are presented as mean ± SEM. P values were calculated using the Student t test. ****P < .001; ***P < .005.