The promoter region and the −17 kb URE of the PU.1 gene are highly methylated in cHL cells. (A) Bisulfite sequencing confirmed that the promoter of PU.1 is highly methylated in L428 and KM-H2 Hodgkin lymphoma cell lines. ○ represents methylated cytosines. Underlining indicates the PU.1 binding site downstream of the translation initiation site. (B) Bisulfite sequencing revealed that the −17 kb URE was highly methylated in L428 and KM-H2 Hodgkin lymphoma cell lines. ○ represents methylated cytosines. Underlining indicates 3 RUNX1 (AML1) binding sites and a PU.1 binding site.